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alkali to alter the pH to a value where the enzyme is inactive are usually effective but methods involving transfer of the reaction vessel to an ice or boiling-water bath may be less satisfactory if the volume of the assay mixture is relatively large.
substrates in ice to ensure their stability.
Figure 8 demonstrates a comparison of a solid-phase and solution-phase assay for interleukin converting enzyme (ICE).
Figure 8 Time-course analysis of an interleukin-converting-enzyme (ICE) assay.
and 'on' bead formats using 1 mg streptavidin-coated PVT SPA beads. 0.27 Ci [3H]ICE substrate was
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